Publications

Publications


2021

139. Jutras, P. V., Dodds, I., and Van der Hoorn, R. A. L. (2021) A bioluminescent Agrobacterium tumefaciens for imaging bacterial metabolic activity in planta. Meth. Mol. Biol. in press.

138. Buscaill, P., and Van der Hoorn, R. A. L. (2021) Monitoring Pseudomonas syringae growth in agroinfiltrated leaves: the ‘agromonas’ assay. Meth. Mol. Biol. in press.

137. Schuster, M., Paulus, J. K., Kourelis, J., and Van der Hoorn, R. A. L. (2021) Purification of His-tagged proteases from the apoplast of agroinfiltrated N. benthamiana. Meth. Mol. Biol. in press.

136. Morimoto, K., Stegmann, M., Kaschani, F., Mohammed, S., and Van der Hoorn, R. A. L. (2021) Cysteine reactivity profiling to unveil redox regulation in phytopathogens. Meth. Mol. Biol. in press.

135. Van der Hoorn, R. A. L. and Klemenčič, M. (2021) Plant Proteases: from molecular mechanisms to functions in development and immunity. J. Exp. Bot. 72, 3337-3339. pubmed

134. Buscaill, P. and Van der Hoorn, R. A. L. (2021) Defeated by the nines: nine extracellular strategies to avoid MAMP recognition in plants. Plant Cell in press. pubmed

133. Woude, L.V., Piotrowski, M., Klaasse, G., Paulus, J.K., Krahn, D., Ninck, S., Kaschani, F., Kaiser, M., Novák, O., Ljung, K., Bulder, S., van Verk, M., Snoek, B.L., Fiers, M., Martin, N.I., Van der Hoorn, R.A.L., Robert, S., Smeekens, S., and Van Zanten, M. (2021) The chemical compound 'Heatin' stimulates hypocotyl elongation and interferes with the Arabidopsis NIT1-subfamily of nitrilases. Plant J. in press. pubmed

132. Godson, A., and Van der Hoorn, R. A. L. (2021) The frontline of defence: meta-analysis of apoplastic proteases in plant immunity. J. Exp. Bot. 72, 3381-3394. pubmed

131. Wang, S., Xing, R., Wang, Y., Shi, H., Fu, S., Huang, J., Paulus, J. K., Schuster, M., Saunders, D. G. O., Win, J., Vleeshouwers, V., Wang, Y., Zheng, X., Van der Hoorn, R. A. L., and Dong, S. (2021) Cleavage of a pathogen apoplastic protein by plant subtilases activates host immunity. New Phytol. 229, 3424-3439. pubmed

130. Buscaill, P., Sanguankiattichai, N., Lee, Y. J., Kourelis, J., Preston, G., and Van der Hoorn, R. A. L. (2021) Agromonas: a rapid disease assay for Pseudomonas syringae growth in agroinfiltrated leaves. Plant J. 105, 831-840. pubmed

2020

129. Parry, G., Benitez-Alfonso, Y., Gibbs, D. J., Grant, M., Harper, A., Harrison, C. J., Kaiserli, E., Leonelli, S., May, S., McKim, S., Spoel, S., Turnbull, C., Van der Hoorn, R. A. L. and Murray, J. (2020) How to build an effective research network: lessons from two decades of the GARNet plant science community. J. Exp. Bot. 71, 6881-6889. pubmed

128. Kourelis, J., Malik, S., Mattinson, O., Krauter, S., Kahlon, P. S., Paulus, J. K., and Van der Hoorn, R. A. L. (2020) Evolution of a guarded decoy protease and its receptor in solanaceous plants. Nat. Comm. 11, 4393 pubmed

127. Paulus, J. K., Kourelis, J., Ramasubramanian, S., Homma, F., Godson, A., Hoerger, A. C., Hong, T. N., Krahn, D., Ossorio Carballo, L., Wang, S., Win, J., Smoker, M., Kamoun, S., Dong, S., and Van der Hoorn, R. A. L. (2020) Extracellular proteolytic cascade in tomato activates immune protease Rcr3. Proc. Nat. Acad. Sci. USA 117, 17409-17417. pubmed

126. Schuster, M., and Van der Hoorn, R. A. L. (2020) Distinct new players in processing peptide hormones during abscission. Curr. Biol. 30, R715-R717. pubmed

125. Bach-Pages, M., Homma, F., Kourelis, J., Kaschani, F., Mohammed, S., Kaiser, M., Van der Hoorn, R. A. L., Castello, A., and Preston, G. M. (2020) Discovering the RNA-binding proteome of plant leaves with an improved RNA interactome capture method. Biomolecules 10, 661. pubmed

124. Minina, E. A., Staal, J., Alvarez, V. E, Berges, J. A., Berman-Frank, I., Beyaert, R., Bidle, K. D., Bornancin, F., Casanova, M., Cazzulo, J. J., Choi, C. J., Coll, N. S., Dixit, V. M., Dolinar, M., Fasel, N., Funk, C., Gallois, P., Gevaert, K., Gutierrez-Beltran, E., Hailfinger, S., Klemencic, M., Koonin, E. V., Krappmann, D., Linusson, A., Machado, M. F. M., Madeo, F., Megeney, L. A., Moschou, P. N., Mottram, J. C., Nyström, T., Osiewacz, H. D., Overall, C. M., Pandey, K. C., Ruland, J., Salvesen, G. S., Shi, Y., Smertenko, A., Stael, S., Ståhlberg, J., Suárez, M. F., Thome, M., Tuominen, H., Van Breusegem, F., Van der Hoorn, R. A. L., Vardi, A., Zhivotovsky, B., Lam, E., and Bozhkov, P. V. (2020) Classification and nomenclature of metacaspases and paracaspases: no more confusion with caspases. Mol. Cell 77, 927-929. pubmed

123. Kriechbaum, R., Ziaee E., Grünwald-Gruber, C., Buscaill, P., Van der Hoorn, R. A. L., and Castilho, A. (2020) BGAL1 depletion boosts the level of β-galactosylation of N- and O-glycans in N. benthamiana. Plant Biotechn. J. 18, 1537-1549. pubmed

122. Jutras, P. V., Dodds, I., and Van der Hoorn, R. A. L. (2020) Proteases of Nicotiana benthamiana: an emerging battle for molecular pharming. Curr. Opin. Biotechn. 61, 60-65. pubmed

2019

121. Kourelis, J., Kaschani, F., Grosse-Holz, F. M., Homma, F., Kaiser, M., and Van der Hoorn, R. A. L. (2019) A homology-guided, genome-based proteome for improved proteomics in the alloploid Nicotiana benthamiana. BMC Genomics 20, 722. pubmed

120. Morimoto, K., Cole, K. S., Kourelis, J., Witt, C. H., Brown, D., Krahn, D., Stegmann, M., Kaschani, F., Kaiser, M., Burton, J., Mohammed, S., Yamaguchi-Shinozaki, K., Weerapana, E., and Van der Hoorn, R. A. L. (2019) Triazine probes target ascorbate peroxidases in plants. Plant Physiol. 180, 1848-1859. pubmed

119. Morimoto, K., and Van der Hoorn, R. A. L. (2019) Plant Biology: proteolytic release of danger signals. Curr. Biol. 29, 378-390. pubmed

118. Buscaill, P., Chandrasekar, B., Sanguankiattichai, N., Kourelis, J., Kaschani, F., Thomas, E. L., Morimoto, K., Kaiser, M., Preston, G. M., Ichinose, Y., and Van der Hoorn, R. A. L. (2019) Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides. Science 364, 145. pubmed

117. Fernandez-Fernandez, A. D., Van der Hoorn, R. A. L., Gevaert, K., Van Beusegem, F., and Stael, S. (2019) Caught green-handed: methods for in vivo detection and visualization of protease activity. J. Exp. Bot. 70, 2125-2141. pubmed

116. Vinegra de la Torre, N., Kaschani, F., Kaiser, M., Van der Hoorn, R. A. L., Soppe, W. J. J., and Misas-Villamil, J. C. (2019) Dynamic hydrolase labelling as a marker for seed quality in Arabidopsis seeds. Biochem. J. 476, 843-857. pubmed

115. Pogorelko, P., Juvale, P., Rutter, W., Hütten, M., Maier, T., Hewezi, T., Paulus, J. K., Van der Hoorn, R. A. L., Grundler, F., Siddique, S., Lionetti, V., Zabotina, O., Baum, T. (2019) Retargeting of a defence protease by a nematode effector. Plant J. 98, 1000-1014. pubmed

114. Jutras, P. V. , Grosse-Holz, F., Kaschani, F., Kaiser, M., Michaud, D., and Van der Hoorn, R. A. L. (2019) Activity-based proteomics reveals nine target proteases for the recombinant protein-stabilising inhibitor SlCYS8 in Nicotiana benthamiana. Plant Biotechn. J. 17, 1670-1678. pubmed

113. Poret, M., Chandrasekar, B., Dechaumet S., Bouchereau, A., Kim, T. H., Lee, B. R., Macquart F., Hara-Nishimura I, Van der Hoorn, R. A. L., Avice, J. C. (2019) A genotypic comparison reveals that the improvement in nitrogen remobilisation efficiency in oilseed rape leaves is related to specific patterns of senescence-associated protease activities and phytohormones. Front Plant Sci. 10, 46. pubmed

112. Paulus, J. K. and Van der Hoorn, R. A. L. (2019) Do proteolytic cascades exist in plants? J. Exp. Bot. 70, 1997-2002. pubmed

111. Santos, R. B., Pires, A. S., Van der Hoorn, R. A. L., Schiermeyer, A., and Abranches, R. (2018) Generation of transgenic cell suspension cultures of the model legume Medicago truncatula: a rapid method for Agrobacterium-mediated gene transfer. Plant Cell & Organ Culture (PCTOC) 13, e1800050. pubmed

110. Ormancey, M., Thuleau P., Van der Hoorn, R. A. L., Grat, S., Testard, A., Kamal, K. Y., Boudsocq, M., Cotelle, V., and Mazars, C. (2018) Shingolipid-induced cell death in Arabidopsis is negatively regulated by the papain-like cysteine protease RD21. Plant Sci.280, 12-17. pubmed

2018

109. Bravo Cadena, M., Preston, G. M., Van Der Hoorn, R. A. L., Flanagan, N. A., Townley, H. E., & Thompson, I. P. (2018). Enhancing cinnamon essential oil activity by nanoparticle encapsulation to control seed pathogens. Ind. Crops &Products 124, 755–764. ORA

108. Bravo Cadena, M., Preston, G. M., Van der Hoorn, R. A. L., Townley, H. E., Thompson, I. P. (2018) Species-specific antimicrobial activity of essential oils and enhancement by encapsulation in mesoporous silica nanoparticles. Ind. Crops & Products 122, 582-590. ORA

107. Van der Hoorn, R. A. L. and Rivas, S. (2018) Unravelling the role of plant proteases. New Phytol. 218, 879-881. pubmed

106. Clark, K., Franco, J., Schwizer, S., Pang, Z., Hawara, E., Liebrand, T., Pagliaccia, D., Zeng, L., Gurung, F., Wang, P., Shi, J., Wang, Y., Ancona, V., Van der Hoorn, R. A. L., Wang, N., Coaker, G., and Ma, W. (2018) An effector from the Huanglongbing-associated pathogen targets citrus proteases. Nat. Comm. 9, 1718 pubmed

105. Planas, M., Bernardo-Faura, M., Paulus, J. K., Kaschani, F., Kaiser, M., Valls, M., Van der Hoorn, R. A. L., and Coll, N. (2018) Protease activities triggered by Ralstonia solanacearum infection in susceptible and tolerant tomato lines. Mol. Cell. Proteomics 17, 1112-1125. pubmed

104. Santos, R. B., Chandrasekar, B., Mandal, M. K., Kaschani, F., Kaiser, M., Both, L., Van der Hoorn, R. A. L., Schiermeyer, A., Abranches R.(2018) Low protease content in Medicago truncatula cell cultures facilitates recombinant protein production. Biotechn. J. 13, e1800050. pubmed

103. Husaini, A. M., Morimoto, K., Chandrasekar, B., Kelly, S., Kaschani, F., Palmero, D., Jiang, J., Kaiser, M., Ahrazem, O., Overkleeft, H. S., and Van der Hoorn, R. A. L. (2018) Multiplex fluorescent activity profiling of α-glycosidases in plants. Plant Physiol. 177, 24-37. pubmed

102. Grosse-Holz, F., Madeira, L., Zahid, M. A., Songer, M., Kourelis, J., Fesenko, M., Ninck, S., Kaschani, F., Kaiser, M. and Van der Hoorn, R. A. L. (2018) Three unrelated protease inhibitors enhance accumulation of pharmaceutical recombinant proteins in N. benthamiana. Plant Biotechn. J.16, 1797-1810. pubmed

101. Thomas, E. L., and Van der Hoorn, R. A. L. (2018) Ten prominent host protease in plant-pathogen interactions. Int. J. Mol. Sci. 19, 639. pubmed

100. Kourelis, J., and Van der Hoorn, R. A. L. (2018) Defended to the nines: 25 years of resistance gene cloning identifies nine mechanisms of R protein function. Plant Cell 30, 285-299. pubmed

99. Paulus, J. K. and Van der Hoorn, R. A. L. (2018) Tricked or Trapped - two decoy mechanisms in host-pathogen interactions. PLoS Pathog. 14, e1006761. pubmed

2017

98. Zhang, H., Gannon, L., Hassall, K., Deery, M., Gibbs, D., Holdsworth, M., Van der Hoorn, R. A. L., Lilley, K., and Theodoulou, F. (2017) N-terminomics reveal control of Arabidopsis seed storage proteins and proteases by the Arg/N-end rule pathway. New Phytol. 218, 1106-1126. pubmed

97. Grosse-Holz, F., Kelly, S., Blaskowski, S., Kaschani, F., Kaiser, M., and Van der Hoorn, R. A. L. (2017) The transcriptome, extracellular proteome and active secretome of agroinfiltrated N. benthamiana uncover a large, diverse protease repertoire. Plant Biotechn. J. 16, 1068-1084. pubmed

96. Poret, M., Chandrasekar, B., Van der Hoorn, R. A. L., Coquet, L., Jouenne, T., and Avice, J. -C. (2017) Proteomic investigations of proteases involved in cotyledon senescence: a model to explore the genotypic variability of proteolysis machinery associated with nitrogen remobilisation efficiency during leaf senescence of oilseed rape. Proteomes 5, 29. pubmed

95. Teper-Bamnolker, P., Buskila, Y., Belausov, E., Wolf, D., Doron-Faigenboim, A., Ben-Dor, S., Van der Hoorn, R. A. L., Lers, A., Eshel, D. (2017) Vacuolar processing enzyme (VPE) activates programmed cell death in the apical meristem inducing loss of apical dominance. Plant Cell & Environ. 40, 2381-2392. pubmed

+++ Lu, H., Chandrasekar, B., Oeljeklaus, J., Misas-Villamil, J. C., Wang, Z., Shindo, T., Bogyo, M., Kaiser, M., and Van der Hoorn, R. A. L. Correction in Plant Physiol. 174, 465-466 on Ref.72 link

+++ Van der Hoorn, R. A. L., Rivas, S., Wulff, B. B. H., Jones, J. D. G., and Joosten, M. H. A. J. Correction in Plant J. 90, 1212 on Ref.11 link

94. Schaller, A., Stintzi, A., Rivas, S., Serano, I., Sueldo, D. J., Van der Hoorn, R. A. L., Ramirez, V., Vera, P. (2017) From structure to function - a family portrait of plant subtilases. New Phytol. 218, 901-915. pubmed

93. Paulus, J. K., Kourelis, J., and Van der Hoorn, R. A. L. (2017) Bodyguards: pathogen-derived decoys that protect virulence factors. Trends Plant Sci. 22, 355-357. pubmed

92. Sueldo, D. J., and Van der Hoorn, R. A. L. (2017) Plant life needs cell death, but does plant cell death need Cys proteases? FEBS Lett. 284, 1577-1585. pubmed

91. Kovacs, J., Poor, P., Kaschani, F., Chandrasekar, B., Hong, T. N., Misas-Villamil, J. C., Xin, B. T., Kaiser, M., Overkleeft, H. S., Tari, I., and Van der Hoorn, R. A. L. (2017) Proteasome activity profiling uncovers alteration of catalytic beta-2 and beta-5 subunits of the stress-induced proteasome during salinity stress in tomato roots. Frontiers Plant Sci. 8, 107. pubmed

90. Misas, J. C., Van der Burgh, A., Grosse-Holz, F., Bach-Pages, M., Kaschani, F., Schilasky, S., Emon, A. E. K., Ruben, M., Kaiser, M., Overkleeft, H. S., and Van der Hoorn, R. A. L. (2017) Subunit-selective proteasome activity profiling uncovers uncoupled proteasome subunit activities during bacterial infections. Plant J. 90, 418-430. pubmed

89. Pruzinska, A., Shindo, T., Niessen, S., Kaschani, F., Toth, R., Millar, A. H., and Van der Hoorn, R. A. L. (2017) Major Cys protease activities are not essential for senescence in individually darkened Arabidopsis leaves. BMC Plant Biol. 17, 4. pubmed

88. Chandrasekar, B., Hong, T. N., and Van der Hoorn, R. A. L. (2017) Inhibitor discovery by convolution ABPP. Meth. Mol. Biol. 1491, 47-56. pubmed

2016

87. Shindo, T., Kaschani, F., Yang, F., Kovacs, J., Tian, F., Kourelis, J., Hong, T. N., Colby, T., Shabab, M., Chawla, R., Kumari, S., Ilyas, M., Hoerger, A., Alfano, J. R., and Van der Hoorn, R. A. L. (2016) Screen of non-annotated small secreted proteins of Pseudomonas syringae reveals a virulence factor that inhibits tomato immune proteases. PLoS Pathog. 12, e1005874. pubmed

86. Misas-Villamil, J. C., Van der Hoorn, R. A. L., and Doehlemann, G. (2016) Papain-like cysteine proteases as hubs in plant immunity. New Phytol. 212, 902-907. pubmed

85. Daneri-Castro, S. N., Chandrasekar, B., Grosse-Holz, F. M., Van der Hoorn, R. A. L., and Roberts, T. H. (2016) Activity-based protein profiling of hydrolytic enzymes induced by gibberellic acid in isolated aleurone layers of malting barley. FEBS Lett. 590, 2956-2962. pubmed

84. Paireder, M., Mehofer, U., Tholen, S., Porodko, A., Schahs, P., Biniossek, M. L., Van der Hoorn, R. A. L., Lenarcic, B., Novinec, M., Schilling, O., and Mach, L. (2016) The death enzyme CP14 is a unique papain-like cysteine proteinase with a pronounced S2 subsite selectivity. Arch. Biochem. Biophys. 603, 110-107. pubmed

83. Kovacs, J., and Van der Hoorn, R. A. L. (2016) Twelve ways to confirm targets of activity-based probes in plants. Bioorg. Med. Chem. 24, 3304-3311. pubmed

++ Van der Hoorn, R. A. L. (2016) Exploring antagonistic interactions in the plant apoplast. Biology News (Oxford University) 5, 11. pdf

82. Kaschani, F., Wei, Q., Dingemans, J., Van der Hoorn, R. A. L., Cornelis, P., and Kaiser, M. (2016) Capture of endogenously biotinylated proteins from Pseudomonas auruginosa displays unexpected regulation of LiuD upon iron nutrition. Bioorg. Med. Chem. 24, 3330-3335. pubmed

81. Kourelis, J., Van der Hoorn, R. A. L., Sueldo, D. J. (2016) Decoy engineering: the next step in resistance breeding. Trends Plant Sci. 21, 371-373. pubmed

80. Stiti, N., Chandrasekar, B., Strubl, L., Mohammed, S., Bartels, D., and Van der Hoorn, R. A. L. (2016) Nicotinamide cofactors suppress active-site labeling of aldehyde dehydrogenases. ACS Chem. Biol. 11, 1578-1586. pubmed

79. Malik, S., and Van der Hoorn, R. A. L. (2016) Inspirational decoys: a new hunt for effector targets. New Phytologist 210, 371-373. pubmed

78. Poret, M., Chandrasekar, B., Van der Hoorn, R. A. L., and Avice, J. -B. (2016) Characterization of senescence-associated protease activities in the efficient protein remobilization during leaf senescence of winter oilseed rape. Plant Sci. 246, 139-153. pubmed

77. Morimoto, K., and Van der Hoorn, R. A. L. (2016) The increasing impact of activity-based protein profiling in plant science. Plant Cell Physiol. 57, 446-461 pubmed

76. Grosse-Holz, F., and Van der Hoorn, R. A. L. (2016) Juggling jobs: roles and mechanisms of multifunctional protease inhibitors in plants. New Phytologist 57, 446-461. pubmed

75. Chandrasekar, B., and Van der Hoorn, R. A. L. (2016) Beta-galactosidases in Arabidopsis and tomato: a mini review. Biochem. Transact. 44, 150-158. pubmedPDF

2015

74. Hütten, M., Geukes, M., Misas-Villamil, J. C., van der Hoorn, R. A. L., Grundler, F. M., and Siddique, S. (2015) Activity profiling reveals changes in the diversity and activity of proteins in Arabidopsis roots in response to nematode infection. Plant Physiol Biochem. 97, 36-43. pubmed

73. Ilyas, M., Hörger, A. C., Bozkurt, T. O., Van den Burg, H. A., Kaschani, F., Kaiser, M., Belhaj, K., Smoker, M., Joosten, M. H. A. J., Kamoun, S., and Van der Hoorn, R. A. L. (2015) Functional divergence of two secreted immune proteases of tomato. Current Biol. 25, 2300-2306. pubmed

72. Lu, H., Chandrasekar, B., Oeljeklaus, J., Misas-Villamil, J. C., Wang, Z., Shindo, T., Bogyo, M., Kaiser, M., and Van der Hoorn, R. A. L. (2015) Subfamily-specific probes for Cys proteases display dynamic protease activities during seed germination. Plant Physiol. 168, 1462-1475pubmed

71. Ting, H. M., Delatte, T. L., Kolkman, P., Misas-Villamil, J. C., Van der Hoorn, R. A. L., Bouwmeester, H. J., and Van der Krol, A. R. (2015) SNARE-RNAi results in higher terpene emission from ectopically expressed caryophyllene synthase in Nicotiana benthamiana. Mol. Plant 8, 454-466pubmed

2014

++ Van der Hoorn, R. A. L. (2014) Determining the fate of proteins. International Innovation 141, 22-24. PDF website

70. Chandrasekar, B., Colby, T., Emon, A. E. K., Jiang, J., Hong, T. N., Villamor, J. G., Harzen, A., Overkleeft, H. S., and Van der Hoorn, R. A. L. (2014) Broad range glycosidase activity profiling. Mol. Cell. Proteomics. 13, 2787-2800. pubmed

69. Zhang, B., Tremousaygue, D., Denancé, N., Van Esse, H. P., Hörger, A. C., Dabos, P., Goffner, D., Thomma, B. P. H. J., Van der Hoorn, R. A. L., Tuominen, H. (2014) PIRIN2 stabilizes cysteine protease XCP2 and increases susceptibility to the vascular pathogen Ralstonia solanacearum in Arabidopsis. Plant J. 79, 1009-1019. pubmed

68. Sueldo, D., Ahmed, A., Misas-Villamil, J. C., Colby, T., Tameling, W., Joosten, M. H. A. J., and Van der Hoorn, R. A. L. (2014) Dynamic hydrolase activities precede hypersensitive tissue collapse in tomato seedlings. New Phytologist 203, 913-925. pubmed

67.Dong, S., Stam, R., Cano, L. M., Song, J., Sklenar, J., Yoshida, K., Bozkurt, T. O., Oliva, R., Liu, Z., Tian, M., Win, J., Banfield, M. J., Jones, A. M., Van der Hoorn, R. A. L., and Kamoun, S. (2014) Effector specialization in a lineage of the Irish potato famine pathogen. Science 343, 552-555. pubmed PDF Highlighted in Science, The Scientist, Science Daily, Smallholder, SeedDailyBBSRC, PhysOrg, HispanicBusiness, Science Codex, PlantSci, RedOrbit, Vegetable Farmer, Agrotimes, Solar Daily, and Oxford.

66. Hong, T. N., and Van der Hoorn, R. A. L. (2014) DIGE-ABPP by click chemistry: pairwise comparison of serine hydrolase activities from the apoplast of infected plants. Meth. in Mol. Biol. 1127, 183-194. pubmed

2013

65. Ilyas, S., Ilyas, M., Van der Hoorn, R. A. L., and Mathur, S. (2013) Selective conjugation of proteins in mining the active proteome through click-functionalized magnetic nanoparticles. ACS Nano 7, 9655-9663. pubmed

64. Hörger, A. C., and Van der Hoorn, R. A. L. (2013) The structural basis of specific protease-inhibitor interactions at the plant-pathogen interface. Curr. Opin. Struct. Biol. 23, 843-850. pubmed

63. Zulet, A., Gil-Monreal, M., Villamor, J. G., Zabalza, A., Van der Hoorn, R. A. L., and Royuela, M. (2013) Proteolytic pathways induced by herbicides that inhibit amino acid biosynthesis. PLoS One 8, e73847. pubmed

62. Villamor, J. G., Kaschani, F., Colby, T., Oeljeklaus, J., Zhao, D., Kaiser, M., Patricelli, M. P., and Van der Hoorn, R. A. L. (2013) Profiling protein kinases and other ATP binding proteins in Arabidopsis using acyl-ATP probes. Mol. Cell. Proteomics 12, 2481-2496. pubmed

61. Misas-Villamil, J. C., Kolodziejek, I., Crabill, E., Kaschani, F., Niessen, S., Shindo, T., Kaiser, M., Alfano, J. R., and Van der Hoorn, R. A. L. (2013) Pseudomonas syringae pv. syringae uses proteasome inhibitor Syringolin A to colonize from wound infection sites. PLoS Pathogens. 9, e1003281. pubmed

60. Gu, C., Shannon, A., Colby, T., Wang, Z., Shabab, M., Kumari, S., Villamor, J. G., McLaughlin, C. J., Weerapana, E., Kaiser, M., Cravatt, B. F., and Van der Hoorn, R. A. L. (2013) Chemical proteomics with sulfonyl fluoride probes reveals selective labeling of functional tyrosines in glutatione transferases. Chem. & Biol. 20, 541-548pubmed PDF Highlighted by Dr. Verhelst in same issue!

59. Nguyen, H. M., Schippers, J. H., Goni-Ramos, O., Christoph, M. P., Dortay, H., Van der Hoorn, R. A. L., and Mueller-Roeber, B. (2013) An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana. Plant J. 74, 25-36. pubmed

58. Lu, H., Wang, Z., Shabab, M., Oeljeklaus, J., Verhelst, S. H., Kaschani, F., Kaiser, M., Bogyo, M., and Van der Hoorn, R. A. L. (2013) A substrate-inspired probe monitors translocation, activation and subcellular targetting of bacterial type III effector protease AvrPphB. Chem. & Biol. 20, 168-176. pubmed Highlighted by Dr. Blum in same issue!

57. Misas-Villamil, J. C., Toenges, G., Kolodziejek, I., Sadaghiani, A. M., Kaschani, F., Colby, T., Bogyo, M., and Van der Hoorn, R. A. L. (2013) Activity profiling of vacuolar processing enzymes reveals a role for VPE during oomycete infection. Plant J. 73, 689-700. pubmed

++. Van der Hoorn, R. A. L. RD21 Peptidase. Book chapter in Handbook of proteolytic enzymes, 3rd edition. (N. Rawings & G. Salvesen, eds.), Elsevier 2013, 1892–1896. link

 2012

56. Stolze*, S. C., Deu* E., Kaschani*, F., Li, N., Florea, B. J., Richau, K. H., Colby, T., Van der Hoorn, R. A. L., Overkleeft, H. S., Bogyo, M., and Kaiser, M. (2012) The antimalarial natural product Symplostatin 4 is a nanomolar inhibitor of the food vacuole Falcipains. Chem. & Biol. 19, 1546-1555. pubmed

55. Shannon, D., A., Gu, C., McLaughlin, C. J., Kaiser, M., Van der Hoorn*, R. A. L., and Weerapana*, E. (2012) Sulfonyl-fluoride analogs as activity-based probes for serine proteases. ChemBioChem, 13, 2327-2330. pubmed

54. Van der Linde, K., Mueller, A. N., Hemetsberger, C., Kaschani, F., Van der Hoorn, R. A. L., and Doehlemann, G. (2012) The maize cystatin CC9 interacts with apoplastic cysteine proteases. Plant Sign. Behav. 7, 1397-1401. pubmed

53. Hörger, A. C., Ilyas, M., Stephan, W., Tellier, A., Van der Hoorn*, R. A. L. and Rose*, L. E. (2012) Balancing selection at the tomato RCR3 guardee gene family maintains variation in strength of pathogen defense. PLoS Genetics 8, e1002813. pubmed PDF

52. Lozano-Torres, J. L., Wilbers, R. H. P., Gawronski, P., Boshoven, J. C., Finkers-Tomczak, A., Cordewener, J. H. G., America, A. H. P., Overmars, H. A., Van 't Klooster, J. W., Baranowski, L., Sobczak, M., Ilyas, M., Van der Hoorn, R. A. L., Schots, A., De Wit, P. J. G. M., Bakker, J., Goverse, A., and Smant, G. (2012) Dual disease resistance mediated by immune receptor Cf-2 in tomato require a common virulence target of a fungus and a nematode. Proc. Nat. Acad. Sci. USA 109, 10119-10124. pubmed

51. Richau, K., Kaschani, F., Verdoes, M., Pansuriya, T. C., Niessen, S., Stüber, K., Overkleeft, H. S., Bogyo, M., and Van der Hoorn, R. A. L. (2012) Subclassification and biochemical analysis of plant papain-like cysteine proteases displays subfamily-specific characteristics. Plant Physiol. 158, 1583-1599. pubmed

50. Van der Linde, K., Hemetsberger, C., Kastner, C., Kaschani, F., Van der Hoorn, R. A. L., Kumlehn, J., and Doehlemann, G. (2012) A maize cystatin suppresses host immunity by inhibition of apoplastic cysteine proteases. Plant Cell 24, 1285-1300. pubmed

49. Kaschani, F., Clerc, J., Krahn, D., Ottmann, C., Bier, D., Ngoc Hong, T., Ottmann, C., Niessen, S., Colby, T., Van der Hoorn, R. A. L., and Kaiser, M. (2012) Identification of a selective, activity-based probe for glyceraldehyde 3-phosphate dehydrogenases. Angew. Chem. Int. Ed. 51, 5230-5233. pubmed

48. Gu, C., Shabab, M., Strasser, R., Wolters, P. J., Shindo, T., Niemer, M., Kaschani, F., Mach, L., and Van der Hoorn, R. A. L. (2012) Post-translational regulation and trafficking of the granulin-containing protease RD21 of Arabidopsis thaliana. PLoS One 7, e32422. pubmed PDF

47. Ottmann, C., Van der Hoorn, R. A. L., and Kaiser, M. (2012) The impact of plant-pathogen studies on medicinal drug discovery. Chem. Soc. Rev. 41, 3168-3178 pubmed

46. Kaschani, F., Gu, C., and Van der Hoorn, R. A. L. (2012) Activity-based protein profiling of infected plants. Meth. Mol. Biol. 835, 47-59. pubmed PDF

45. Shindo, T., Misas-Villamil, J. C., Hörger, A., Song, J., and Van der Hoorn, R. A. L. (2012) A role in immunity for Arabidopsis cysteine protease RD21, the ortholog of the tomato immune protease C14. PLoS One 7, e29317. pubmed

44. Kaschani, F., Nickel, S., Pandey, B., Cravatt, B.F., Kaiser, M., and Van der Hoorn, R. A. L. (2012) Selective inhibition of plant serine hydrolases by agrochemicals revealed by competitive ABPP. Bioorg. Med. Chem. 20, 597-600. pubmed PDF

43. Lenger, J., Kaschani, F., Lenz, T., Dalhoff, C., Villamor, J. G., Koster, H., Sewald, N., and Van der Hoorn, R. A. L. (2012) Labeling and enrichment of Arabidopsis thaliana matrix metalloproteases using an active-site directed, marimastat-based photoreactive probe. Bioorg. Med. Chem.20, 592-596. pubmed 

42. Nickel, S., Kaschani, F., Colby, T., Van der Hoorn, R. A. L., and Kaiser, M. (2012) A para-nitrophenol phosphonate probe labels distinct serine hydrolases in Arabidopsis. Bioorg. Med. Chem. 20, 601-606. pubmed

++. Van der Hoorn, R. A. L., Gu, C., Kaschani, F., Misas-Villamil, J. C., Richau, K., Kolodziejek, I., Moquet, F., Malliart, C., Berthet, X., Goni-Ramos, O., Ellul, P. (2012) Seed trait prediction by activity-based protein profiling. WO Patent 2012100963

2011 

41. Bozkurt, T., Schornack, S., Win, J., Shindo, T., Ilyas, M., Oliva, R., Cano, L. M., Jones, A. M. E., Huitema, E., Van der Hoorn, R. A. L., and Kamoun, S. (2011) Phytophthora infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface. Proc. Natl. Acad. Sci USA. 108, 20832-20837. pubmed

40. Van der Hoorn, R. A. L., and Kaiser, M. (2012) Probes for activity-based profiling of plant proteases. Physiol. Plantarum 145, 18-27. pubmed

39. Van der Hoorn, R. A. L., Colby, T., Nickel, S., Richau, K., Schmidt, J., and Kaiser, M. (2011) Mining the active proteome of Arabidopsis thaliana. Frontiers Plant Physiol. 2, 89. pubmed

38. Kumari, S., and Van der Hoorn, R. A. L. (2011) A structural biology perspective on bioactive small molecules and their plant targets. Curr. Opin. Plant Biol. 14, 480-488. pubmed

37. Misas-Villamil*, J. C., Kolodziejek*, I., and Van der Hoorn, R. A. L. (2011) Pseudomonas syringae colonizes distant tissues in Nicotiana benthamiana through xylem vessels. Plant J. 67, 774-782. pubmed

36. Kaschani, F., and Van der Hoorn, R. A. L. (2011) A model of the C14-EPIC complex indicates hotspots for a protease-inhibitor arms race in the oomycete-potato interaction. Plant Signal. Behav. 6, 1-4. pubmed

35. Kolodziejek*, I., Misas-Villamil*, J. C., Kaschani, F., Clerc, J., Gu, C., Krahn, D., Niessen, S., Verdoes, M., Willems, L. I., Overkleeft, H. S., Kaiser, M. and Van der Hoorn, R. A. L. (2011) Proteasome activity imaging and profiling characterizes bacterial effector Syringolin A. Plant Physiol. 155, 477-489. pubmed

34. Richau, K. H., and Van der Hoorn, R. A. L. (2011) Studies on plant-pathogen interactions using activity-based proteomics. Curr. proteomics 7, 328-336. website

33. Lenz, H. D., Haller, E., Melzer, E., Kober, K., Wurster, K., Stahl, M., Bassham, D. C., Vierstra, R. D., Parker, J. E., Bautor, J., Molina, A., Escudero, V., Shindo, T., Van der Hoorn, R. A. L., Gust, A. A., and Nuernberger, T. (2011) Autophagy differentially controls plant basal immunity to biotrophic and necrotrophic pathogens. Plant J. 66, 818-830. pubmed

2010

32. Kaschani, F., Shabab, M., Bozkurt, T., Shindo, T., Schornack, S., Gu, C., Ilyas, M., Win, J., Kamoun, S., and Van der Hoorn, R. A. L. (2010) An effector-targeted protease contributes to defense against Phytophthora infestans and is under diversifying selection in natural hosts. Plant Physiol. 154, 1794-1804. pubmed

++. Van der Hoorn, R. A. L., Gu, C., Ilyas, M., Kolodziejek, I., Kumari, S., Misas-Villamil, J. C., Pruzinska, A., Richau, K. H., Shabab, M., Shindo, T., Wang, Z. and Kaschani, F. (2010) Mining the active proteome in plant-pathogen interactions. In: Biology of Plant-Microbe Interactions, Volume 7. H. Antound, T. Avis, L. Brisson, D. Prevost, and M. Trepanier, eds. International Society for Molecular Plant-Microbe Interactions, St. Paul, MN.

31. Kolodziejek, I., and Van der Hoorn, R. A. L. (2010) Mining the active plant proteome in plant science and biotechnology. Curr. Opin. Biotechn. 21, 225-233. pubmed

30. Gu, C., Kolodziejek, I., Misas-Villamil, J. C., Shindo, T., Colby, T., Verdoes, M., Richau, K. H., Schmidt, J., Overkleeft, H. S., and Van der Hoorn, R. A. L. (2010) Proteasome activity profiling: a simple, robust and versatile method revealing subunit-selective inhibitors and cytoplasmic, defence-induced proteasome activities. Plant J. 62, 160-170. pubmed PDF

29. Toth, R., and Van der Hoorn, R. A. L. (2010) Emerging principles in plant chemical genetics. Trends in Plant Sci. 15, 81-88. pubmed

2009

28. Kaschani*, F., Gu*, C., Niessen, S., Hoover, H., Cravatt, B. F., and Van der Hoorn, R. A. L. (2009) Diversity of Serine hydrolase activities of non-challenged and Botrytis-infected Arabidopsis thaliana. Mol. Cell. Proteomics 8, 1082-1093 pubmed PDF

27. Song, J., Win, J., Tian, M., Schornack, S., Kaschani, F., Muhammad, I., Van der Hoorn, R. A. L., and Kamoun, S. (2009) Apoplastic effectors secreted by two unrelated eukaryotic plant pathogens target the tomato defense protease Rcr3. Proc. Natl. Acad. Sci. USA 106, 1654-1659. pubmed

26. Hogenhout, S. A., Van der Hoorn, R. A. L., Terauchi, R., and Kamoun, S. (2009) Emerging concepts in effector biology of plant-associated organisms. Mol. Plant-Microbe Interact. 22, 115-122.pubmed

25. Kaschani, F., Verhelst, S. H. L., Van Swieten, P. F., Verdoes, M., Wong, C. -S., Wang, Z., Kaiser, M., Overkleeft, H. S., Bogyo, M., and Van der Hoorn, R. A. L. (2009) Minitags for small molecules: detecting targets of reactive small molecules in living plant tissues using 'click-chemistry'. Plant J.57, 373-385. pubmed PDF

++. Van der Hoorn, R. A. L. Activity-based protein profiling of Cys proteases. Book chapter in: Chemical Biology. Learning through case studies. (H. Waldmann, P. Janning, eds.) Wiley VCH, 2009, pp69-78.

2008

24. Van der Hoorn, R. A. L., and Kamoun, S. (2008) From guard to decoy: a new model for perception of plant pathogen effectors. Plant Cell 20, 2009-2017. pubmed

23. Wang*, Z., Gu*, C., Colby, T., Shindo, T., Balamurugan, R., Waldmann, H., Kaiser, M., and Van der Hoorn, R. A. L. (2008) Beta-lactone probes identify a papain-like peptide ligase in Arabidopsis thaliana. Nat. Chem. Biol. 4, 557-563. pubmed  Highlighted by Carrico I., Nat. Chem. Biol. (2008) pressrelease (German)

22. Misas-Villamil, J. C., and Van der Hoorn, R. A. L. (2008) Enzyme-inhibitor interactions at the plant-pathogen interface. Curr. Opin. Plant Biol. 11, 380-388 pubmed

21. Shabab*, M., Shindo*, T., Gu, C., Kaschani, F., Pansuriya, T., Chintha, R., Harzen A., Colby, T., Kamoun, S., and Van der Hoorn, R. A. L. (2008) Fungal effector protein AVR2 targets diversifying defence-related Cys proteases of tomato. Plant Cell 20, 1169-1183. pubmed

20. Van der Hoorn, R. A. L. (2008) Plant proteases: from phenotypes to molecular mechanisms. Ann. Rev. Plant Biol. 59, 191-223. pubmed PDF

19. Shindo, T., and Van der Hoorn, R. A. L. (2008) Papain-like cysteine proteases: key players at molecular battlefields employed by both plants and their invaders. Mol. Plant Pathol. 9, 119-125. MPP

2007

18. Gilroy, E., Hein, I., Van der Hoorn, R. A. L., Boevink, P., Venter, E., McLellan, H., Kaffarnik, F., Hrubikova, K., Shaw, J., Holeva, M., Lopez, E., Hidalgo, O., Pritchard, L., Loake, G., Lacomme, C., and Birch, P. (2007) Involvement of cathepsin B in the plant disease resistance hypersensitive response. Plant J. 52, 1-13. pubmed

++. Kaschani, F., and Van der Hoorn, R. A. L. (2007) Anwendung von activity-based proteomics zur Analyse von Pflanzen-Pathogen Interaktionen. Max Planck Society Yearbook 2007, 625-629. link

17. Kaschani, F., and Van der Hoorn, R. A. L. (2007) Small molecule approaches in plants. Curr. Opin. Chem. Biol. 11, 88-98. pubmed

16. Tian, M., Win, J., Song, J., Van der Hoorn, R. A. L., Van der Knaap, E., and Kamoun, S. (2007) A Phytophthora infestans cystatin-like protein interacts with and inhibits a tomato papain-like apoplastic protease. Plant Physiol. 143, 364-277. pubmed

 2005

15. Rooney*, H., Van ’t Klooster*, J., Van der Hoorn, R. A. L., Joosten, M. H. A. J., Jones, J. D. G., and De Wit, P. J. G. M. (2005) Cladosporium Avr2 inhibits tomato Rcr3 protease required for Cf-2-dependent disease resistance. Science 308, 1783-1789. pubmed
Recommended in faculty 1000.

14. Van der Hoorn*, R. A. L., Wulff*, B., Rivas*, S., Durrant, M. C., Van der Ploeg, A., De Wit, P. J. G. M., and Jones, J. D. G. (2005) Structure-function analysis of Cf-9, a receptor-like protein with extracytoplasmic leucine-rich repeats. Plant Cell 17, 1000-1015. pubmed

2004

13. Van der Hoorn, R. A. L. and Jones, J. D. G. (2004) The plant proteolytic machinery and its role in defence. Curr. Opin. Plant Biol. 7, 400-407. pubmed

12. Van der Hoorn, R. A. L., Leeuwenburgh, M. A, Bogyo, M., Joosten, M. H. A. J., and Peck, S. C. (2004) Activity profiling of papain-like cysteine proteases in plants. Plant Physiol. 135, 1170-1178. pubmed Highlighted in faculty 1000.

2003

11. Van der Hoorn, R. A. L., Rivas, S., Wulff, B. B. H., Jones, J. D. G., and Joosten, M. H. A. J. (2003). Rapid migration in gel filtration of the Cf-4 and Cf-9 resistance proteins is an intrinsic property of Cf proteins and not because of their association with high-molecular-weight proteins. Plant J. 35, 305-315. pubmed

2002

10. Van der Hoorn, R. A. L., De Wit, P. J. G. M., and Joosten, M. H. A. J. (2002) Balancing selection favors guarding resistance proteins. Trends Plant Sci. 6, 67-71. pubmed

2001

9. Van der Hoorn, R. A. L., Roth, R., and De Wit, P. J. G. M. (2001) Identification of distinct specificity determinants in resistance protein Cf-4 allows construction of a Cf-9 mutant that confers recognition of avirulence protein AVR4. Plant Cell 13, 273-285. pubmed

8. Van der Hoorn, R. A. L., Van der Ploeg, A., De Wit, P. J. G. M., and Joosten, M. H. A. J. (2001) The C-terminal dilysine motif for targeting to the endoplasmic reticulum is not required for Cf-9 function. Mol. Plant-Microbe Interact. 14, 412-415. pubmed

7. Van der Hoorn*, R. A. L., Kruijt*. M., Roth, R., Brandwagt, B. F., Joosten, M. H. A. J., and De Wit, P. J. G. M. (2001) Intragenic recombination generated two distinct Cf genes that mediate AVR9 recognition in the natural population of Lycopersicon pimpinellifolium. Proc. Natl. Acad. Sci. USA 98, 10493-10498. pubmed

6. Joosten, M. H. A. J., Brandwagt, B. F., Van der Hoorn, R. A. L., De Jong, C. F., Van 't Klooster, J. W., De Kock, M. J. D., Kruijt, M., Luderer, R., Takken, F. L. W., Westerink, N. and De Wit, P. J. G. M. (2001) The role of secreted proteins of the pathogenic fungus Cladosporium fulvum in (a) virulence on its host, tomato. Phytopathology 91, S161.

5. De Wit, P. J. G. M., Brandwagt, B. F., Van den Burg, H. A., Cai, X., Van der Hoorn, R. A. L., De Jong, C. F., Van't Klooster, J., De Kock, M. J. D., Kruijt, M., Lindhout, W. H., Luderer, R., Takken, F. L. W., Westerink, N., Vervoort, J. J. M., Joosten, M. H. A. J. (2001) The molecular basis of co-evolution between Cladosporium fulvum and tomato. Anton. Leeuw. Int. J. G. 81, 409-412. pubmed

2000

4. Van der Hoorn, R. A. L., Laurent, F., Roth, R., and De Wit, P. J. G. M. (2000) Agroinfiltration is a versatile tool that facilitates comparative analysis of Avr9/Cf-9-induced and Avr4/Cf-4-induced necrosis. Mol. Plant-Microbe Interact. 13,439-446. pubmed

3. Luderer, R., Rivas, S., Nürnberger, T., Mattei, B., Van den Hooven, H. W., Van der Hoorn, R. A. L., Romeis, T., Wehrfritz, J. M., Blume, B., Nennstiel, D., Zuidema, D., Vervoort, J., De Lorenzo, G., Jones, J. D. G., De Wit, P. J. G. M., and Joosten, M. H. A. J. (2000) No evidence for binding between resistance gene product Cf-9 of tomato and avirulence gene product AVR9 of Cladosporium fulvum. Mol. Plant-Microbe Interact. 14, 867-876. pubmed

2. Stam, M., De Bruin, R., Van Blokland, R., Van der Hoorn, R. A. L., Mol, J. N. M., and Kooter, J. M. (2000) Distinct features of post-transcriptional gene silencing by antisense transgenes in single copy and inverted T-DNA repeat loci. Plant J. 21, 27-42. pubmed

++. Joosten, M. H. A. J., Cai, X. Z., Van der Hoorn, R. A. L., De Jong, C. F., De Kock, M., Lauge, R., Luderer, R., Roth, R., Takken, F., Vossen, P., Weide, R., Westerink, N., De Wit, P. J. G. M. (2000) Fungal (A)virulence and host resistance in the Cladosporium fulvum-tomato interaction. Book chapter in: Biology of Plant-Microbe Interactions. Vol.2, APS-PRESS 2000, 29-34.

1997

1. Stam, M., De Bruin, R., Kenter, S., Van der Hoorn, R. A. L., Van Blokland, R., Mol, J. N. M., and Kooter, J. M. (1997) Post-transcriptional silencing of chalcone synthase in Petunia by inverted transgene repeats. Plant J. 12, 63-82.

*, equal contibutions; underlined, teammember during contribution

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Research

Research


Our four main research themes

Our research is centered around investigating the roles of apoplastic (extracellular) hydrolases. From a plant biology perspective we study the manipulation of apoplastic hydrolases by microbes and unravel pathogen recognition in the apoplast, focussed on Rcr3 and Cf-2 of tomato. From the technology perspective we pioneer ABPP in plant science and use this to study hydrolase activities and provide a collaboration platform for the plant science community. From the perspective of applied science, we control the activities of apoplastic proteases to improve molecular pharming.

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Plant Chemetics Laboratory

University of Oxford

Welcome to the Plant Chemetics Laboratory


The Plant Chemetics lab investigates the molecular mechanisms underpinning host manipulation by plant pathogens, with a particular focus on apoplast manipulation of Solanaceous plants by bacterial microbes. Our research activities also aim at improved molecular pharming of secreted (glyco)proteins through the depletion of extracellular proteases. Unique is our approach to display protein activities by pioneering activity-based protein profiling (ABPP), a powerful functional proteomics technology that offers an exciting platform for collaborations. Please contact us if you are interested to join or collaborate!

News

Interview plant chemetics lab


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News

News


  • May 2021

    Defeated by the nines: nine extracellular strategies to avoid MAMP recognition in plants. An impressive review on how plant pathogens avoid recognition in the plant apoplast, published in The Plant Cell. Read here more about the first author Pierre Buscaill.

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  • April 2021

    ERC Advanced grant! We will soon be spending 2,5 million euro to explore the plant-pathogen interface with chemical proteomics! Postdoc positions will be advertised soon!

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  • March 2021

    Special Issue on Plant Proteases with an impressive review by Alice Godson with a meta-analysis of secreted immune proteases in plants!

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  • December 2020

    Emma has submitted her D. Phil. thesis and successfully defended this during her viva. After the has made the minor corrections we can call her Dr. Emma! Congrats!!!

  • September 2020

    Evolution of a guarded decoy published in Nature Communications. An impressive piece of research, spear-headed by Jiorgos Kourelis.

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  • July 2020

    First proteolytic cascade in plants: Subtilase P69B of tomato activates immune protease Rcr3. Wonderful protein biochemistry uncovered by Judith Paulus! See more in PNAS.

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  • April 2020

    A new BBSRC grant with Gail Preston to identify the BGAL1 inhibitor produced by Pseudomonas syringae! Exciting years ahead!

  • April 2019

    Our Science manuscript is online! An exciting discovery on the extracellular arms-race at the plant-bacterium interface.

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  • February 2019

    We celebrate the acceptance of Pierre's manuscript! A great discovery made through a great collaboration involving many labmembers. Will be online soon.

  • January 2019

    Happy 2019! New year dinner at Somerville college. Looking back to a successful 2018 and forward to an exciting 2019. Cheers!

  • December 2019

    Best poster prize for Shivani Malik! Truly exceptional, out of 1300 posters!

  • September 2018

    Lab retreat in Wales! With fantastic weather for a long hike, fun games, and an excellent scientific brainstorm.

  • September 2018

    TWO best poster awards for Emma and Kyoko at the 4th conference on Plant Proteases and PCD in Ghent! Well done both!!!

  • June 2018

    A second BBSRC grant! This time on the role of substrates of immune protease Pip1 in the infections by Phytophthora infestans.

  • May 2018

    New BBSRC grant to study the role of extracellular glycosidases in immunity!

  • April 2018

    New probes to monitor active alpha-glycosidases in plants, applied to study anthesis and infection of saffron crocus.

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  • March 2018

    We hosted the 11th International Conference on Activity-based Protein Profiling (ABPP2018) in Somerville College, Oxford: 104 participants, 30 presentations and 35 posters.

  • February 2018

    Plant Biotechn. J.: Three new protease inhibitors that boost recombinant protein expression upon agroinfiltration.

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  • January 2018

    The fruits of 25 years of R gene cloning: 314 R genes group into 9 common overarching mechanisms. Well done, Jiorgos!

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  • November 2017

    Niben secrets unlocked: Friederike Grosse-Holz published her extensive datasets on the transcriptome, extracellular proteome and active secretome of agroinfiltrated Nicotiana benthamiana in Plant Biotechn. J.

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  • November 2017

    Highly Cited Researcher 2017! Declared by Clarivate Analytics (web of science). There are only 15 UK researchers in the field of Plant & Animal Science awarded with this title.

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  • Oktober 2017

    Top personal records at Oxford half marathon! Maria (2:01:23), Judith (1:57:49), and Friederike (1:52:06). Well done, athletes!

  • September 2017

    Best seminar award!

    Jiorgos Kourelis gave the best PhD seminar the Oxford Congress of Plant Sciences!

  • September 2017

    Full Professor in Plant Sciences!

    Renier van der hoorn has been awarded the title of Full Professor in the most recent Recognition of Distinction Exercise at the University of Oxford.

  • Januari 2017

    New EU Grant: 'Newcotiana'

    A new EU grant has been awarded to the Plant Chemetics lab as part of a larget Horizon 2020-funded collaborative grant coined 'Newcotiana' on improving Nicotiana species for Molecular Pharming.

  • Maart 2017

    This video describes the activities in The Plant Chemetics Lab. A special thanks to Dr. Amjad Husaini for putting this together!!!

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  • Maart 2017

    Four new team members run the Teddy Hall Relais under the team name 'Running buffers'! Excellent time!

  • January 2017

    NEW: Subunit-selective proteasome probes. Activity profiling with these probes revealed uncoupling uf subunit activities dring Pseudomonas syrningae infection. For more infomration, See Misas-Villamil et al., Plant J. 2017 pubmed.

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  • November 2016

    Declared a Highly Cited Researcher, according to Thomson Reuters! In the UK, there are only 16 researchers in the field of Plant & Animal Science rewarded with this title.

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  • Oktober 2016

    Three team members have run the Oxford half marathon and broke their personal records. Kyoko finished at at 1:50:43, Judith at 1:56:59 and Marcel at 2:03:29 (partly on one leg). Well done everyone!!!

  • September 2016

    'Springwalk' in the Valley of the White Horse. We had fantastic weather!!!

  • August 2016

    PtoDC3000 produces Cys protease inhibitor Cip1!

    Pseudomonas syringae pv. tomato DC3000 (PtoDC3000) produces an inhibitor targeting secreted Cys proteases of its host, tomato. We coined this chagasin-like inhibitor Cip1 and it evades recognition by the Rcr3/Cf-2 system, possibly by its low affinity for Rcr3.  This is the fourth unrelated pathogen-derived inhibitor targeting the same secreted Cys proteases of the host. Previously identified inhibitors were from a tomato-pathogenic fungus (Avr2), oomycete (EpiCs) and nematode (Vap1).

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  • April 2016

    We organized a exciting Conference on Plant Proteases in Somerville College, on 10-12 April 2016.

    These 86 participants represent >40 research groups and work on diverse topics in plant biology but face common challenges on unravelling the action and substrates of plant proteases.

  • Maart 2016

    John Fell Funding awarded to Plant Chemetics lab

    The John Fell Fund has awarded 75k£ to establish quantitative hyper-reactive cysteine profiling in Oxford and apply this technology to investigate how the proteome of a bacterial plant pathogen responds to a host-generated oxidative burst.

  • February 2016

    In ACS Chemical Biology: cofactor suppresses active site labelling.

    We uncovered a strong correlation between enzymatic activity and active site labelling for aldehyde dehydrogenases, with one remarkable exception: nicotinamide cofactors suppress active site labelling.

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  • November 2015

    Announcement of International Conference on Plant Proteases

    This 3-day conference will be held in the centre of Oxford, April 10-12 2016. For speakers, venues, registration and other information, please visit www.plantproteases2016.com.

  • Oktober 2015

    'Springwalk' in Chiltern.

    A beautiful hike in the autumn forests of the Chilterns to Nettlebed and back, 35mins from Oxford. Some trees were irresistible...

  • Oktober 2015

    Kyoko runs the Oxford half-marathon
     
     
    Kyoko Morimoto broke her personal record by crossing the finish within two hours (at 1:59:59, to be exact). Congratulations!
  • August 2015

    In Current Biology: Functional divergence of two secreted immune proteases of tomato. We show that Rcr3 and Pip1 are paralogous proteases that differ in their expression levels and exposed surface and diverged >36 mya. Pip1 is an important immune protease acting against unrelated apoplastic pathogens, while Rcr3 is a co-receptor for pathogen-derived inhibitors, playing a minor role in the absence of immune receptor Cf-2.

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  • July 2015

    NEW: On-line probe target library. We have assembled all our published probes, experiments and probe targets in a searchable database. Click here to search for labeling conditions, probes or targets.

  • Juni 2015

    In Plant Physiology: NEW probes for Cys protease subfamilies!

    We introduce new fluorescent probes for three subfamilies of Cys proteases: aleurains (ALPs), cathepsin B's (CTBs) and vacuolar processing enzymes (VPEs). These probes are widely applicable and display unexpected dynamics of Cys proteases during seed germination. See Lu et al., Plant Physiol. in press.

  • May 2015

    Fascination of Plants Day

    Judith, Luisa and Bala entertained the public (and themselves) by infiltrating plants during the Fascination of Plants Day (May 18) at the Harcourt Arboretum.

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  • April 2015

    Exciting new projects for university students!

    For biology/chemistry students for periods of 3-9 months on a diverse range of topics and using a broad variety of techniques. Please see the flyer for more information and contact Renier van der Hoorn for more information.

  • Maart 2015

    COST Meeting Chemical Proteomics
    12-14 April 2015, Oxford

    We are organizing the COST Meeting on Chemical Proteomics under the title: 'Mining the Chemical Proteome: Target discovery using chemical probes and their application in medicine and biology'. We have scheduled 25 speakers, 35 poster presentations and have over 100 participants. For more information, please download the flyer or program.

  • Oktober 2014

    GreenProtease project has started!

    This ERC-funded project has started with the arrival of Dr. Luisa Madeira in October. Friederike Grosse-Holz and Dr. Daniela Sueldo will join the GreenProtease team in November and January, respectively. Together they will unravel the proteolytic machinery of Nicotiana benthamiana by protease depletion to improve recombinant (glyco)protein production and elucidate the roles and substrates of the plant proteases. Welcome and success!

  • August 2014

    In MCP: Broad-range glycosidase profiling

    Activity-based probes for glucosidases also label the active sites of dozens of other glycosidases! We show that these probes are broadly applicable on various plants, sub-proteomes and living cells.

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  • Juni 2014

    In New Phytologist: Hydrolases precede hypersensitive tissue collapse.

    Activity-based protein profiling was used to describe the active hydrolase content of tomato seedlings undergoing hypersensitive cell death. Differential hydrolase activities precede tissue collapse and are also found in plants infected with the fungal pathogen Cladosporium fulvum.

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  • January 2014

    In Science: Potato blight lineage adapted to new host proteases.

    A collaboration with the laboratory of Sophien Kamoun (Sainsbury lab, Norwich, UK) resulted in the discovery of the molecular details of host adaptation of a Phytophthora infestans lineage that jumped onto a different host species about 1300 years ago. The protease content of this new host species is different, which forced the pathogen to adapt its protease inhibitors. A single amino acid residue exchange underpins host adaptation and is associated with a reduced ability to manipulate the proteases from the former host. Published in Science. Comments published in Science, The Scientist, and Oxford Media.

  • December 2013

    An ERC-CoG-2013 consolidator grant has been awarded to Dr. Renier van der Hoorn for his 'GreenProteases' proposal with the full title: 'The proteolytic machinery of the plant apoplast: from basic understanding to improved recombinant protein production. The EU wil sponsor this project with 2.038.196 euro for a period of 5 years.

  • September 2013

    In MCP: Monitoring the ATP binding proteome

    Many protein activities are driven by ATP hydrolysis. We monitored ATP binding activities of the Arabidopsis proteome using Acyl-ATP probes that label conserved lysine residues in ATP binding pockets. We discovered that these probes label a diversity of protein kinases, including receptor-like kinases, MAP kinases, calcium-dependent kinases and PTI-like kinases.

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  • March 2013

    In PLoS Pathogens: Pathogen enters wounds by inhibiting host proteasome

    Pseudomonas syringae pv. syringae is an epiphyte that occasionally causes disease upon wounding by hail, frost, strong winds and sand storms. We discovered that these bacteria enter wound sites and colonize adjacent tissues by producing proteasome inhibitor Syringolin A (SylA). SylA diffuses from the primary infections site and blocks salicylic acid (SA) signalling in adjacent tissues, creating an SA-insensitive zone that is ready for colonization.

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  • April 2013

    In Chem & Biol: Probing functional tyrosines

    We discovered that besides serine proteases, probes based on serine protease inhibitor AEBSF also label many other proteins, often at tyrosine residues residing in active sites, indicating that these ‘reactivity probes’ highlight functional tyrosines in proteomes. Indeed, labeling of glutathione S-transferases (GSTs) occurs on a single tyrosine that is not conserved in the substrate binding site but nevertheless essential for GST function.

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  • February 2013

    In Plant J: A new tool for studying vacuolar processing enzymes

    Vacuolar processing enzymes (VPEs) are legumain-like cysteine proteases involved in programmed cell death and protein processing. We introduce a specific, activity-bases probe to detect VPE activities in living tissues of different plants and discovered that VPE activity is upregulated upon infection with an oomycete pathogen. Interestingly, oomycete infection benefits from VPEs, probably by supporting their biotrophic lifestyle.

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  • February 2013

    In Chem & Biol: Tracing bacterial effector in action

    Plant pathogenic bacteria manipulate their host by injecting effector proteins. One of these effectors is AvrPphB, a cysteine protease that cleaves host kinases involved in immunity. Inspired by the unique substrate cleavage specificity of AvrPphB, we designed an activity-based probe that targets this protease. We used this probe to demonstrate that the prodomain of AvrPphB is required for secretion and its removal required of activity in the host cell.

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